J Genomics 2015; 3:82-84. doi:10.7150/jgen.12886

Short Research Communication

Draft Genome Sequences of Two Species of “Difficult-to-Identify” Human-Pathogenic Corynebacteria: Implications for Better Identification Tests

Luis G. C. Pacheco1, ✉, Ana L. Mattos-Guaraldi2, Carolina S. Santos1, Adonney A. O. Veras3, Luis C. Guimarães3, 4, Vinícius Abreu4, Felipe L. Pereira5, Siomar C. Soares5, Fernanda A. Dorella5, Alex F. Carvalho5, Carlos G. Leal5, Henrique C. P. Figueiredo5, Juliana N. Ramos2, 6, Veronica V. Vieira6, Eric Farfour7, Nicole Guiso7, Raphael Hirata Jr.2, Vasco Azevedo4, Artur Silva3, #, Rommel T. J. Ramos3, #, ✉

1. Institute of Health Sciences, Federal University of Bahia (UFBA), Salvador, BA, Brazil.
2. Faculty of Medical Sciences, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil.
3. Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, PA, Brazil.
4. Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
5. National Reference Laboratory for Aquatic Animal Diseases, Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
6. National Institute for Quality Control in Health - Oswaldo Cruz Foundation (INCQS - Fiocruz), Rio de Janeiro, RJ, Brazil.
7. Unité de Prévention et Thérapie Moléculaires des Maladies Humaines, Institut Pasteur, Paris, France.
# These authors share credit for senior authorship in this work.

Abstract

Non-diphtheriae Corynebacterium species have been increasingly recognized as the causative agents of infections in humans. Differential identification of these bacteria in the clinical microbiology laboratory by the most commonly used biochemical tests is challenging, and normally requires additional molecular methods. Herein, we present the annotated draft genome sequences of two isolates of “difficult-to-identify” human-pathogenic corynebacterial species: C. xerosis and C. minutissimum. The genome sequences of ca. 2.7 Mbp, with a mean number of 2,580 protein encoding genes, were also compared with the publicly available genome sequences of strains of C. amycolatum and C. striatum. These results will aid the exploration of novel biochemical reactions to improve existing identification tests as well as the development of more accurate molecular identification methods through detection of species-specific target genes for isolate's identification or drug susceptibility profiling.

Keywords: Corynebacterium spp., Emerging pathogens, Biochemical tests, Molecular identification

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How to cite this article:
Pacheco LGC, Mattos-Guaraldi AL, Santos CS, Veras AAO, Guimarães LC, Abreu V, Pereira FL, Soares SC, Dorella FA, Carvalho AF, Leal CG, Figueiredo HCP, Ramos JN, Vieira VV, Farfour E, Guiso N, Hirata R Jr., Azevedo V, Silva A, Ramos RTJ. Draft Genome Sequences of Two Species of “Difficult-to-Identify” Human-Pathogenic Corynebacteria: Implications for Better Identification Tests. J Genomics 2015; 3:82-84. doi:10.7150/jgen.12886. Available from http://www.jgenomics.com/v03p0082.htm