J Genomics 2016; 4:19-22. doi:10.7150/jgen.16082 This volume
Short Research Communication
1. Section of Developmental Genomics, Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
2. Joint Initiative for Metrology in Biology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
*These authors contributed equally to this work.
Spike-in RNAs are valuable controls for a variety of gene expression measurements. The External RNA Controls Consortium developed test sets that were used in a number of published reports. Here we provide an authoritative table that summarizes, updates, and corrects errors in the test version that ultimately resulted in the certified Standard Reference Material 2374. We have noted existence of anti-sense RNA controls in the material, corrected sub-pool memberships, and commented on control RNAs that displayed inconsistent behavior.
Keywords: ERCC, spike-in controls, external RNA controls, NIST standard reference materials.