J Genomics 2016; 4:19-22. doi:10.7150/jgen.16082 This volume

Short Research Communication

External RNA Controls Consortium Beta Version Update

Hangnoh Lee1*✉, P. Scott Pine2*, Jennifer McDaniel2, Marc Salit2, Brian Oliver1

1. Section of Developmental Genomics, Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
2. Joint Initiative for Metrology in Biology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
*These authors contributed equally to this work.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
Lee H, Pine PS, McDaniel J, Salit M, Oliver B. External RNA Controls Consortium Beta Version Update. J Genomics 2016; 4:19-22. doi:10.7150/jgen.16082. Available from https://www.jgenomics.com/v04p0019.htm

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Graphic abstract

Spike-in RNAs are valuable controls for a variety of gene expression measurements. The External RNA Controls Consortium developed test sets that were used in a number of published reports. Here we provide an authoritative table that summarizes, updates, and corrects errors in the test version that ultimately resulted in the certified Standard Reference Material 2374. We have noted existence of anti-sense RNA controls in the material, corrected sub-pool memberships, and commented on control RNAs that displayed inconsistent behavior.

Keywords: ERCC, spike-in controls, external RNA controls, NIST standard reference materials.