J Genomics 2018; 6:1-8. doi:10.7150/jgen.23248 This volume Cite
Research Paper
1. Animal Genetics and Genomics Laboratory, International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853
2. Department of Animal Science, Berry College, Mount Berry, GA 30149
3. Department of Animal and Dairy Science, University of Georgia, Athens, GA 30602
4. Departamento Regional de Bioingenierias, Instituto Tecnologico y de Estudios Superiores de Monterrey, Queretaro, Mexico
5. Usomi Ltd., PO Box 105086-00101, Ushirika Road, Karen, Nairobi, Kenya
6. Department of Biotechnology, University of Sargodha, Sargodha, Pakistan
7. Department of Molecular Biology, Virtual University of Pakistan, Lahore, Pakistan
8. African Institute for Biosciences Research and Training, Ibadan, Nigeria
9. School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332
10. African Institute for Biosciences Research and Training, Ibadan, Nigeria
11. Department of Biomedical Sciences, Rochester Institute of Technology, Rochester NY, 14623
DNAJA1 or heat shock protein 40 (Hsp40) is associated with heat adaptation in various organisms. We amplified and sequenced a total of 1,142 bp of bovine Hsp40 gene representing the critical N-terminal (NTR) and C-terminal (CTR) regions in representative samples of African, Asian and American cattle breeds. Eleven and 9 different haplotypes were observed in the NTR in Asian and African breeds respectively while in American Brangus, only two mutations were observed resulting in two haplotypes. The CTR appears to be highly conserved between cattle and yak. In-silico functional analysis with PANTHER predicted putative deleterious functional impact of c.161 T>A; p. V54Q while alignment of bovine and human NTR-J domains revealed that p.Q19H, p.E20Q and p. E21X mutations occurred in helix 2 and p.V54Q missense mutation occurred in helix 3 respectively. The 124 bp insertion found in the yak DNAJA1 ortholog may have significant functional relevance warranting further investigation. Our results suggest that these genetic differences may be concomitant with population genetic history and possible functional consequences for climate adaptation in bovidae.
Keywords: Cattle, Hsp40 gene, climate adaptation, genetic diversity