Isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium Pseudomonas sp. JG-B from nylon 11 enrichment culture

Jocelyn Gatz-Schrupp, Peter Deckard, Benjamin Hufford, Steven Ly, Peter Tupa, Hisako Masuda^✉

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Citation:
Gatz-Schrupp J, Deckard P, Hufford B, Ly S, Tupa P, Masuda H. Isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium Pseudomonas sp. JG-B from nylon 11 enrichment culture. J Genomics 2020; 8:16-20. doi:10.7150/jgen.42095. Available from https://www.jgenomics.com/v08p0016.htm

Abstract

Nylon 11 is a polymer synthesized from 11-aminoundecanoic acid, and widely used in commercial manufacturing. In this study, we describe the isolation of the first organism capable of metabolizing 11-aminoundecanoic acid from nylon 11 enrichment culture. The strain shows rapid growth on 11-aminoundecanoic acid as a sole source of carbon, nitrogen, and energy. Furthermore, the genome sequence of strain JG-B was deciphered and shown to belong to genus Pseudomonas. Many genes encoding putative extracellular hydrolases, as well as homologues of nylon 6 hydrolases (NylB and NylA) were identified, suggesting the metabolic versatility and possibility that this organism could also depolymerase nylon 11 polymers.

Keywords: Nylon 11, biodegradation, genome, Pseudomonas, 11-aminoundecanoic acid, hydrolases.