J Genomics 2020; 8:80-83. doi:10.7150/jgen.50468 This volume

Research Paper

Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones

Takashi Kitano✉*, Choong-Gon Kim#, Naruya Saitou

Division of Population Genetics, National Institute of Genetics, Japan.
*Present address: Graduate School of Science and Engineering, Ibaraki University, Japan.
#Present address: Marine Ecosystem Research Center, Korea Institute of Ocean Science and Technology, Korea.

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Kitano T, Kim CG, Saitou N. Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones. J Genomics 2020; 8:80-83. doi:10.7150/jgen.50468. Available from https://www.jgenomics.com/v08p0080.htm

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Graphic abstract

We sequenced the western gorilla (Gorilla gorilla) HoxA cluster region using seven fosmid clones, and found that the total tiling path sequence was 214,185 bp from the 5' non-genic region of HoxA1 to the 3' non-genic region of Evx1. We compared the nucleotide sequence with the gorilla genome sequence in the NCBI database, and the overall proportion of nucleotide difference was estimated to be 0.0005-0.0007. These estimates are lower than overall genomic polymorphism in gorillas.

Keywords: Fosmid library, Gorilla, HoxA