Identification and Characterization of a Potential Antibiotic Producing Strain of Pantoea ananatis

Michael J. LaGier^✉, Mark McDaniel, Alyssa Ragner, Amber Castillo

Department of Biology, Grand View University, Des Moines, IA, USA.

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Citation:
LaGier MJ, McDaniel M, Ragner A, Castillo A. Identification and Characterization of a Potential Antibiotic Producing Strain of Pantoea ananatis. J Genomics 2022; 10:26-32. doi:10.7150/jgen.70066. Available from https://www.jgenomics.com/v10p0026.htm

Abstract

Antibiotic resistance continues to be a significant public health challenge. Soil bacteria represent a potential source of yet to be discovered antimicrobials. The screening of Iowa (United States) soils yielded the identification of a strain of Pantoea ananatis (MMB-1), which displayed an antimicrobial-producing phenotype against a bacterium (Bacillus subtilis) representative of Gram-positive bacteria. Crude, organic, extracts of MMB-1 retained the anti-microbial activity. The draft genome of strain MMB-1 contains a total of 4,634,340 bp, and 4,624 protein-encoding genes. Consistent with phenotypic observation, the genome of MMB-1 encodes for a number of putative secondary metabolite biosynthetic gene clusters, including those known to be involved in the production of the antibiotics lankacidin C and bottromycin. This study increases our overall understanding of Panteoa as a group, and is also consistent with the notion that members of this genus have significant potential as useful natural product producers.

Keywords: Pantoea, antibiotic, soil