J Genomics
2022; 10:49-56.
doi:10.7150/jgen.74788 This volumeCite
Research Paper
Draft Genomes of Nitrogen-fixing Frankia Strains Ag45/Mut15 and AgPM24 Isolated from Root Nodules of Alnus Glutinosa
Philippe Normand1✉, Petar Pujic1, Danis Abrouk1, Spandana Vemulapally2, Trina Guerra2, Camila Carlos-Shanley2, Dittmar Hahn2
1. Université Claude-Bernard Lyon 1, Université de Lyon, UMR 5557 CNRS Ecologie Microbienne, Villeurbanne, France. 2. Texas State University, Department of Biology, 601 University Drive, San Marcos, TX 78666, USA.
✉ Corresponding author: Philippe Normand, Université Claude-Bernard Lyon 1, Université de Lyon, UMR 5557 CNRS Ecologie Microbienne, Villeurbanne, France. E-mail: philippe.normandfr.
Citation:
Normand P, Pujic P, Abrouk D, Vemulapally S, Guerra T, Carlos-Shanley C, Hahn D. Draft Genomes of Nitrogen-fixing Frankia Strains Ag45/Mut15 and AgPM24 Isolated from Root Nodules of Alnus Glutinosa. J Genomics 2022; 10:49-56. doi:10.7150/jgen.74788. https://www.jgenomics.com/v10p0049.htm
The genomes of two nitrogen-fixing Frankia strains, Ag45/Mut15 and AgPM24, isolated from root nodules of Alnus glutinosa are described as representatives of a novel candidate species. Phylogenomic and ANI analyses confirmed that both strains are related to cluster 1 frankiae, and that both strains belong to a novel species. At 6.4 - 6.7 Mb, their genomes were smaller than those of other cultivated Alnus-infective cluster 1 strains but larger than that of the non-cultivated Alnus-infective cluster 1 Sp+ strain AgTrS that was their closest neighbor as assessed by ANI. Comparative genomic analyses identified genes essential for nitrogen-fixation, gene composition as regards COGs, secondary metabolites clusters and transcriptional regulators typical of those from Alnus-infective cluster 1 cultivated strains in both genomes. There were 459 genes present in other cultivated Alnus-infective strains lost in the two genomes, spread over the whole of the genome, which indicates genome erosion is taking place in these two strains.
Normand, P., Pujic, P., Abrouk, D., Vemulapally, S., Guerra, T., Carlos-Shanley, C., Hahn, D. (2022). Draft Genomes of Nitrogen-fixing Frankia Strains Ag45/Mut15 and AgPM24 Isolated from Root Nodules of Alnus Glutinosa. Journal of Genomics, 10, 49-56. https://doi.org/10.7150/jgen.74788.
ACS
Normand, P.; Pujic, P.; Abrouk, D.; Vemulapally, S.; Guerra, T.; Carlos-Shanley, C.; Hahn, D. Draft Genomes of Nitrogen-fixing Frankia Strains Ag45/Mut15 and AgPM24 Isolated from Root Nodules of Alnus Glutinosa. J. Genomics 2022, 10, 49-56. DOI: 10.7150/jgen.74788.
NLM
Normand P, Pujic P, Abrouk D, Vemulapally S, Guerra T, Carlos-Shanley C, Hahn D. Draft Genomes of Nitrogen-fixing Frankia Strains Ag45/Mut15 and AgPM24 Isolated from Root Nodules of Alnus Glutinosa. J Genomics 2022; 10:49-56. doi:10.7150/jgen.74788. https://www.jgenomics.com/v10p0049.htm
CSE
Normand P, Pujic P, Abrouk D, Vemulapally S, Guerra T, Carlos-Shanley C, Hahn D. 2022. Draft Genomes of Nitrogen-fixing Frankia Strains Ag45/Mut15 and AgPM24 Isolated from Root Nodules of Alnus Glutinosa. J Genomics. 10:49-56.
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