Draft Genomes of Symbiotic<i> Frankia</i> Strains AgB32 and AgKG'84/4 from Root Nodules of <i>Alnus Glutinosa</i> growing under Contrasted Environmental Conditions

Normand, Philippe; Pujic, Petar; Abrouk, Danis; Vemulapally, Spandana; Guerra, Trina; Carlos-Shanley, Camila; Hahn, Dittmar

doi:10.7150/jgen.75779

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J Genomics 2022; 10:61-68. doi:10.7150/jgen.75779 This volume Cite

Research Paper

Draft Genomes of Symbiotic Frankia Strains AgB32 and AgKG'84/4 from Root Nodules of Alnus Glutinosa growing under Contrasted Environmental Conditions

Philippe Normand^1✉, Petar Pujic¹, Danis Abrouk¹, Spandana Vemulapally², Trina Guerra², Camila Carlos-Shanley², Dittmar Hahn²

1. Université Claude-Bernard Lyon 1, Université de Lyon, UMR 5557 CNRS Ecologie Microbienne, Villeurbanne, Cedex 69622, France.
2. Texas State University, Department of Biology, 601 University Drive, San Marcos, TX 78666, USA.

Citation:

Normand P, Pujic P, Abrouk D, Vemulapally S, Guerra T, Carlos-Shanley C, Hahn D. Draft Genomes of Symbiotic Frankia Strains AgB32 and AgKG'84/4 from Root Nodules of Alnus Glutinosa growing under Contrasted Environmental Conditions. J Genomics 2022; 10:61-68. doi:10.7150/jgen.75779. https://www.jgenomics.com/v10p0061.htm

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Abstract

The genomes of two nitrogen-fixing Frankia strains, AgB32 and AgKG'84/4, were isolated from spore-containing (spore+) and spore-free (spore-) root nodules of Alnus glutinosa, but they did not sporulate upon reinfection. The two strains are described as representatives of two novel candidate species. Phylogenomic and ANI analyses indicate that each strain represents a novel species within cluster 1, with genome sizes of 6.3 and 6.7 Mb smaller than or similar to those of other cultivated Alnus-infective cluster 1 strains. Genes essential for nitrogen-fixation, clusters of orthologous genes, secondary metabolite clusters and transcriptional regulators analyzed by comparative genomic analyses were typical of those from Alnus-infective cluster 1 cultivated strains in both genomes. Compared to other cultivated Alnus-infective strains with large genomes, those of AgB32 and AgKG'84/4 had lost 380 or 409 genes, among which one hup cluster, one shc gene and the gvp cluster, which indicates genome erosion is taking place in these two strains.

Keywords: Frankia, Actinorhizal symbiosis, genome, nitrogen-fixing frankiae, biosynthetic gene clusters

Citation styles

APA

Normand, P., Pujic, P., Abrouk, D., Vemulapally, S., Guerra, T., Carlos-Shanley, C., Hahn, D. (2022). Draft Genomes of Symbiotic Frankia Strains AgB32 and AgKG'84/4 from Root Nodules of Alnus Glutinosa growing under Contrasted Environmental Conditions. Journal of Genomics, 10, 61-68. https://doi.org/10.7150/jgen.75779.

ACS

Normand, P.; Pujic, P.; Abrouk, D.; Vemulapally, S.; Guerra, T.; Carlos-Shanley, C.; Hahn, D. Draft Genomes of Symbiotic Frankia Strains AgB32 and AgKG'84/4 from Root Nodules of Alnus Glutinosa growing under Contrasted Environmental Conditions. J. Genomics 2022, 10, 61-68. DOI: 10.7150/jgen.75779.

NLM

CSE

Normand P, Pujic P, Abrouk D, Vemulapally S, Guerra T, Carlos-Shanley C, Hahn D. 2022. Draft Genomes of Symbiotic Frankia Strains AgB32 and AgKG'84/4 from Root Nodules of Alnus Glutinosa growing under Contrasted Environmental Conditions. J Genomics. 10:61-68.

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