J Genomics 2014; 2:64-67. doi:10.7150/jgen.7955 This volume
Short Research Communication
1. Committee on Genetics, Genomics, and Systems Biology, The University of Chicago, Chicago IL 60637, USA;
2. Department of Ecology and Evolution, The University of Chicago, Chicago, IL 60637, USA;
3. Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil 05508
Phenotypic differences between males and females of sexually dimorphic species are caused in large part by differences in gene expression between the sexes, most of which occurs in the gonads. To accurately identify genes differentially expressed between males and females in Drosophila, we sequenced the testis and ovary transcriptomes of D. yakuba, D. pseudoobscura, and D. ananassae and used them to identify sex-biased genes in the latter two species. We highlight the increased sensitivity and improved power of sex-biased gene detection methods when using our testis/ovary data versus male and female whole body transcriptome data. We thus provide a resource specifically designed to accurately identify and characterize sex-biased genes across Drosophila. This dataset is available through NCBI GEO accession GSE52058.
Keywords: RNA-seq, testis, ovary, Drosophila pseudoobscura, Drosophila ananassae, Drosophila yakuba