J Genomics 2018; 6:53-62. doi:10.7150/jgen.24788 This volume

Research Paper

Identification of Sex-associated SNPs of Greater Amberjack (Seriola dumerili)

Junya Kawase1✉, Jun-ya Aoki2, Kazuhisa Hamada3, Akiyuki Ozaki2, Kazuo Araki2✉

1. Department of Life Sciences, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, Tsu City, Mie 514-8507, Japan
2. Research Center for Aquatic Breeding, National Research Institute of Aquaculture, Fisheries Research Agency, 224 Hiruda, Tamaki-cho, Watarai, Mie 519-0423, Japan
3. Marine Farm Laboratory Limited Company, 309 Takahiro, Tachibaura, Otsuki-cho, Hata-gun, Kochi 788-0352, Japan

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Kawase J, Aoki Jy, Hamada K, Ozaki A, Araki K. Identification of Sex-associated SNPs of Greater Amberjack (Seriola dumerili). J Genomics 2018; 6:53-62. doi:10.7150/jgen.24788. Available from https://www.jgenomics.com/v06p0053.htm

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Graphic abstract

The sex determination systems of fish are highly diverse compared with those of mammals. Thus, performing investigations using nonmodel fish species helps to understand the highly diverse sex determination systems of fish. Because greater amberjack (Seriola dumerili) is one of the most important edible fish globally and knowledge of its sex determination system is economically important in the field of aquaculture, we are interested in the mechanisms of sex determination of Seriola species. In this study, we identified sex-associated SNPs of greater amberjack using SNP information of 10 males and 10 females by an association test. We determined that the sex-associated SNPs were on chromosome 12 and mainly covered with two scaffolds (about 7.1 Mbp). Genotypes of sex-associated SNPs indicated that females are the heterogametic sex (ZZ/ZW). Furthermore, we compared the genomic structure of greater amberjack with those of Japanese amberjack (Seriola quinqueradiata), California yellowtail (Seriola dorsalis), and medaka (Oryzias latipes). Whole-genome alignments and synteny analysis indicated that the sex determination system of greater amberjack is markedly different from that of medaka and implied that the sex determination system is conserved in the Seriola species.

Keywords: sex determination system, genome-wide association study, heterogametic female, synteny, Seriola dumerili