J Genomics 2018; 6:127-132. doi:10.7150/jgen.27864 This volume

Short Research Paper

De novo male gonad transcriptome draft for the marine mussel Perumytilus purpuratus with a focus on its reproductive-related proteins

Carolina Briones1✉, José J. Nuñez1, Montse Pérez2, Daniela Espinoza-Rojas3, Cristian Molina-Quiroz4, Ricardo Guiñez5

1. Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Casilla 567, Valdivia, Chile
2. Instituto Español de Oceanografía, Centro Oceanográfico de Vigo, 36200 Vigo, España
3. Dirección de Calidad de Pregrado, Universidad de Talca, 2 Norte 685, Talca, Chile
4. Lircaytech, Irarrázabal 3782, Ñuñoa, Santiago de Chile
5. Instituto de Ciencias Naturales Alexander von Humboldt, Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta, Casilla 170, Antofagasta, Chile

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Briones C, Nuñez JJ, Pérez M, Espinoza-Rojas D, Molina-Quiroz C, Guiñez R. De novo male gonad transcriptome draft for the marine mussel Perumytilus purpuratus with a focus on its reproductive-related proteins. J Genomics 2018; 6:127-132. doi:10.7150/jgen.27864. Available from https://www.jgenomics.com/v06p0127.htm

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Graphic abstract

Perumytilus purpuratus is a marine mussel considered a bioengineer species with a broad distribution in the Pacific and Atlantic coast of South America. Studies have shown two geographically and genetically differentiated subpopulations at molecular level and in sperm morphological traits. To open avenues for molecular research on P. purpuratus, a global de novo transcriptome from gonadal tissue of mature males was sequenced using the Illumina platform. From a total of 126.38 million reads, 37,765 transcripts were successfully annotated. BUSCO analysis determined a level of 89% completeness for the assembled transcriptome. The functional gene ontology (GO) annotation indicated that, in terms of abundance, the transcripts related with molecular function were the most represented, followed by those related with biological process and cellular components. Additionally, a subset of GO annotations generated using the “sperm” term resulted in a total of 1,294 sequences where the biological process category was the more represented, with transcripts strongly associated to sperm-processes required for fertilization, and with processes where the sperm-egg interaction could be implicated.

Our work will contribute to the evolutionary understanding of the molecular mechanisms related to tissue-specific functions. This work reports the first male gonad transcriptome for the mussel P. purpuratus, generating a useful transcriptomic resource for this species and other closely related mytilids.

Keywords: Gene Ontology, Mollusca, Bivalve, Sperm, Gamete-recognition proteins, BUSCO