Draft genome sequences for three unisolated Alnus-infective Frankia Sp+ strains, AgTrS, AiOr and AvVan, the first sequenced Frankia strains able to sporulate in-planta

Lorine Bethencourt¹, Florian Vautrin¹, Najwa Taib², Audrey Dubost¹, Lucia Castro-Garcia², Olivier Imbaud², Danis Abrouk¹, Pascale Fournier¹, Jérôme Briolay³, Agnès Nguyen⁴, Philippe Normand¹, Maria P. Fernandez¹, Céline Brochier-Armanet², Aude Herrera-Belaroussi^1✉

1. Univ Lyon, Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR 1418, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France
2. Univ Lyon, Université Lyon 1, CNRS, UMR5558, Laboratoire de Biométrie et Biologie Évolutive, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France
3. Univ Lyon, Université Lyon 1, DTAMB, FR 3728 BioEnviS, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France
4. Biofidal, 170 av Gabriel Péri, F-69518 Vaulx-en-Velin, France

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Citation:
Bethencourt L, Vautrin F, Taib N, Dubost A, Castro-Garcia L, Imbaud O, Abrouk D, Fournier P, Briolay J, Nguyen A, Normand P, Fernandez MP, Brochier-Armanet C, Herrera-Belaroussi A. Draft genome sequences for three unisolated Alnus-infective Frankia Sp+ strains, AgTrS, AiOr and AvVan, the first sequenced Frankia strains able to sporulate in-planta. J Genomics 2019; 7:50-55. doi:10.7150/jgen.35875. Available from https://www.jgenomics.com/v07p0050.htm

Abstract

Actinobacteria from genus Frankia are able to form symbiotic associations with actinorhizal plants including alders. Among them, Sp+ strains are characterized by their ability to differentiate numerous sporangia inside host plant cells (unlike “Sp-” strains unable of in-planta sporulation). Here, we report the first genome sequences of three unisolated Sp+ strains: AgTrS, AiOr and AvVan obtained from Alnus glutinosa, A. incana and A. alnobetula (previously known as viridis), respectively (with genome completeness estimated at more than 98%). They represent new Frankia species based on Average Nucleotide Identity (ANI) calculations, and the smallest Alnus-infective Frankia genomes so far sequenced (~5 Mbp), with 5,178, 6,192 and 5,751 candidate protein-encoding genes for AgTrS, AiOr and AvVan, respectively.

Keywords: Frankia, AgTrS, AiOr, AvVan